제품 > 항체 > 다중클론항체(pAb)

L3MBTL2 Rabbit pAb (A10331)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - L3MBTL2 Rabbit pAb (A10331)

Western blot analysis of extracts of various cell lines, using L3MBTL2 antibody (A10331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameL3MBTL2 Rabbit pAb
Catalog No.A10331
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables methylated histone binding activity. Predicted to be involved in negative regulation of transcription, DNA-templated. Predicted to act upstream of or within several processes, including ectoderm development; regulation of histone modification; and stem cell proliferation. Located in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 486-705 of human L3MBTL2 (NP_113676.2).
SequencePATFCQKNDIELTPPKGYEAQTFNWENYLEKTKSKAAPSRLFNMDCPNHGFKVGMKLEAVDLMEPRLICVATVKRVVHRLLSIHFDGWDSEYDQWVDCESPDIYPVGWCELTGYQLQPPVAAEPATPLKAKEATKKKKKQFGKKRKRIPPTKTRPLRQGSKKPLLEDDPQGARKISSEPVPGEIIAVRVKEEHLDVASPDKASSPELPVSVENIKQETDD
Gene ID
Swiss Prot
SynonymsL3MBT; H-l(3)mbt-l
Calculated MW79kDa
Observed MW95kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, HL-60, HT-29
Cellular locationNucleus

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ABclonal:Western blot - L3MBTL2 Rabbit pAb (A10331)}

Western blot - L3MBTL2 Rabbit pAb (A10331)

Western blot analysis of extracts of various cell lines, using L3MBTL2 antibody (A10331) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)