제품 > 항체 > 단일클론항체(mAb)

LIN28A Rabbit mAb (A21261)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - LIN28A Rabbit mAb (A21261)

Western blot analysis of various lysates using LIN28A Rabbit mAb (A21261) at 1:60000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - LIN28A Rabbit mAb (A21261)

Immunohistochemistry analysis of paraffin-embedded Human embryonic carcinoma using LIN28A Rabbit mAb (A21261) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LIN28A Rabbit mAb (A21261)

Immunohistochemistry analysis of paraffin-embedded Human placenta using LIN28A Rabbit mAb (A21261) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - LIN28A Rabbit mAb (A21261)

Immunohistochemistry analysis of paraffin-embedded Human seminoma using LIN28A Rabbit mAb (A21261) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - LIN28A Rabbit mAb (A21261)

Confocal imaging of HeLa cells using LIN28A Rabbit mAb (A21261, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Overview

Product nameLIN28A Rabbit mAb
Catalog No.A21261
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53062
This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-209 of human LIN28A (NP_078950.1).
SequenceSAKGLESIRVTGPGGVFCIGSERRPKGKSMQKRRSKGDRCYNCGGLDHHAKECKLPPQPKKCHFCQSISHMVASCPLKAQQGPSAQGKPTYFREEEEEIHSPTLLPEAQN
Gene ID
Swiss Prot
SynonymsCSDD1; LIN28; LIN-28; ZCCHC1; lin-28A; LIN28A
Calculated MW23kDa
Observed MW28kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:70000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesT-47D, NCCIT
Cellular locationCytoplasm, Nucleus, nucleolus.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - LIN28A Rabbit mAb (A21261)}

Western blot - LIN28A Rabbit mAb (A21261)

Western blot analysis of various lysates using LIN28A Rabbit mAb (A21261) at 1:60000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunohistochemistry - LIN28A Rabbit mAb (A21261)}

Immunohistochemistry - LIN28A Rabbit mAb (A21261)

Immunohistochemistry analysis of paraffin-embedded Human embryonic carcinoma using LIN28A Rabbit mAb (A21261) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LIN28A Rabbit mAb (A21261)}

Immunohistochemistry - LIN28A Rabbit mAb (A21261)

Immunohistochemistry analysis of paraffin-embedded Human placenta using LIN28A Rabbit mAb (A21261) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - LIN28A Rabbit mAb (A21261)}

Immunohistochemistry - LIN28A Rabbit mAb (A21261)

Immunohistochemistry analysis of paraffin-embedded Human seminoma using LIN28A Rabbit mAb (A21261) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - LIN28A Rabbit mAb (A21261)}

Immunofluorescence - LIN28A Rabbit mAb (A21261)

Confocal imaging of HeLa cells using LIN28A Rabbit mAb (A21261, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)