제품 > 항체 > 단일클론항체(mAb)

LPP Rabbit mAb (A19226)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LPP Rabbit mAb (A19226)

Western blot analysis of various lysates using LPP Rabbit mAb (A19226) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - LPP Rabbit mAb (A19226)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using LPP Rabbit mAb (A19226) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - LPP Rabbit mAb (A19226)

Immunofluorescence analysis of NIH/3T3 cells using LPP Rabbit mAb (A19226) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameLPP Rabbit mAb
Catalog No.A19226
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2381
This gene encodes a member of a subfamily of LIM domain proteins that are characterized by an N-terminal proline-rich region and three C-terminal LIM domains. The encoded protein localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility. This protein also shuttles through the nucleus and may function as a transcriptional co-activator. This gene is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human LPP (Q93052).
SequenceMSHPSWLPPKSTGEPLGHVPARMETTHSFGNPSISVSTQQPPKKFAPVVAPKPKYNPYKQPGGEGDFLPPPPPPLDDSSALPSISGNFPPPPPLDEEAFK
Gene ID
Swiss Prot
SynonymsLPP; lipoma-preferred partner
Calculated MW66kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Hep G2
Cellular locationCell junction, Cell membrane, Cytoplasm, Nucleus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - LPP Rabbit mAb (A19226)}

    Western blot - LPP Rabbit mAb (A19226)

    Western blot analysis of various lysates using LPP Rabbit mAb (A19226) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - LPP Rabbit mAb (A19226)}

    Immunohistochemistry - LPP Rabbit mAb (A19226)

    Immunohistochemistry analysis of paraffin-embedded human liver cancer using LPP Rabbit mAb (A19226) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - LPP Rabbit mAb (A19226)}

    Immunofluorescence - LPP Rabbit mAb (A19226)

    Immunofluorescence analysis of NIH/3T3 cells using LPP Rabbit mAb (A19226) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A19226? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)