제품 > 항체 > 다중클론항체(pAb)

LRFN3 Rabbit pAb (A14927)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - LRFN3 Rabbit pAb (A14927)

Western blot analysis of various lysates using LRFN3 Rabbit pAb (A14927) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameLRFN3 Rabbit pAb
Catalog No.A14927
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in regulation of presynapse assembly; regulation of synaptic membrane adhesion; and synaptic membrane adhesion. Predicted to be located in plasma membrane. Predicted to be active in cell surface and glutamatergic synapse. Predicted to be integral component of postsynaptic density membrane and integral component of presynaptic active zone membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 380-530 of human LRFN3 (NP_078785.1).
SequenceELTVGPPPPPQLANSTSCDPPRDGDPDALTPPSAASASAKVADTGPPTDRGVQVTEHGATAALVQWPDQRPIPGIRMYQIQYNSSADDILVYRMIPAESRSFLLTDLASGRTYDLCVLAVYEDSATGLTATRPVGCARFSTEPALRPCGAP
Gene ID
Swiss Prot
SynonymsSALM4; FIGLER1; LRFN3
Calculated MW66kDa
Observed MW66kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHT-29, Mouse testis, Mouse small intestine, Rat brain
Cellular locationCell junction, Cell membrane, Cell projection, Single-pass type I membrane protein, axon, dendrite, postsynaptic cell membrane, presynaptic cell membrane, synapse
    ABclonal:Western blot - LRFN3 Rabbit pAb (A14927)}

    Western blot - LRFN3 Rabbit pAb (A14927)

    Western blot analysis of various lysates using LRFN3 Rabbit pAb (A14927) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)