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Legumain (LGMN) Rabbit mAb (A6829)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - Legumain (LGMN) Rabbit mAb (A6829)

Western blot analysis of various lysates using Legumain (LGMN) Rabbit mAb (A6829) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameLegumain (LGMN) Rabbit mAb
Catalog No.A6829
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1864
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 210-302 of human Legumain (LGMN) (Q99538).
SequenceANPRESSYACYYDEKRSTYLGDWYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNTSHVMQYGNKTISTMKVMQFQGMKRKASSPVPLPPVTHL
Gene ID
Swiss Prot
SynonymsAEP; LGMN1; PRSC1; Legumain (LGMN)
Calculated MW49kDa
Observed MW36kDa/56kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain, Mouse spleen, Mouse kidney, Rat kidney
Cellular locationLysosome.
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

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ABclonal:Western blot - Legumain (LGMN) Rabbit mAb (A6829)}

Western blot - Legumain (LGMN) Rabbit mAb (A6829)

Western blot analysis of various lysates using Legumain (LGMN) Rabbit mAb (A6829) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)