제품 > 항체 > 단일클론항체(mAb)

Ly-6A/E (Sca-1) Rabbit mAb (A23478)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of various lysates, using Ly-6A/E (Sca-1) Rabbit mAb (A23478) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	Ly-6A/E (Sca-1) Rabbit mAb
Catalog No.	A23478
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC53416

Background

Predicted to enable acetylcholine receptor binding activity and acetylcholine receptor inhibitor activity. Acts upstream of or within response to bacterium. Located in external side of plasma membrane. Is expressed in alimentary system; hindlimb tendon; liver; metanephros; and physiological umbilical hernia. Used to study osteoporosis. Orthologous to human LY6H (lymphocyte antigen 6 family member H).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 27-111 of human Ly-6A/E (Sca-1) (NP_034868.1).
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1; Ly-6A/E (Sca-1)
Calculated MW	14kDa
Observed MW	15kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse lung, Mouse spleen, Mouse kidney
Cellular location	external side of plasma membrane, plasma membrane