제품 > 항체 > 다중클론항체(pAb)

MAFA Rabbit pAb (A18662)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - MAFA Rabbit pAb (A18662)

Western blot analysis of lysates from Mouse brain, using MAFA Rabbit pAb (A18662) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameMAFA Rabbit pAb
Catalog No.A18662
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 269-353 of human MAFA (NP_963883.2).
SequenceQSCRFKRVQQRHILESEKCQLQSQVEQLKLEVGRLAKERDLYKEKYEKLAGRGGPGSAGGAGFPREPSPPQAGPGGAKGTADFFL
Gene ID
Swiss Prot
SynonymsINSDM; hMafA; RIPE3b1; MAFA
Calculated MW37kDa
Observed MW37kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular locationnucleus.

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ABclonal:Western blot - MAFA Rabbit pAb (A18662)}

Western blot - MAFA Rabbit pAb (A18662)

Western blot analysis of lysates from Mouse brain, using MAFA Rabbit pAb (A18662) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)