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MAGE-1/MAGE1A Rabbit mAb (A0723)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - MAGE-1/MAGE1A Rabbit mAb (A0723)

Western blot analysis of extracts of various cell lines, using MAGE-1/MAGE1A Rabbit mAb (A0723) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product nameMAGE-1/MAGE1A Rabbit mAb
Catalog No.A0723
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1839
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-309 of human MAGE-1/MAGE1A (P43355).
SequenceVMIAMEGGHAPEEEIWEELSVMEVYDGREHSAYGEPRKLLTQDLVQEKYLEYRQVPDSDPARYEFLWGPRALAETSYVKVLEYVIKVSARVRFFFPSLREAALREEEEGV
Gene ID
Swiss Prot
SynonymsCT1.1; MAGE1
Calculated MW34kDa
Observed MW51kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, A549, A375, Mouse lung, Mouse testis
Cellular locationCytoplasm, Nucleus

Documents

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ABclonal:Western blot - MAGE-1/MAGE1A Rabbit mAb (A0723)}

Western blot - MAGE-1/MAGE1A Rabbit mAb (A0723)

Western blot analysis of extracts of various cell lines, using MAGE-1/MAGE1A Rabbit mAb (A0723) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

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