제품 > 항체 > 다중클론항체(pAb)

MAGE-1/MAGE1A Rabbit pAb (A5470)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from 22Rv1 cells, using MAGE-1/MAGE1A Rabbit pAb (A5470) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - MAGE-1/MAGE1A Rabbit pAb (A5470)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MAGE-1/MAGE1A Rabbit pAb (A5470) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using MAGE-1/MAGE1A Rabbit pAb (A5470) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded Human stomach cancer using MAGE-1/MAGE1A Rabbit pAb (A5470) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - MAGE-1/MAGE1A Rabbit pAb (A5470)

Immunofluorescence analysis of HeLa cells using MAGE-1/MAGE1A Rabbit pAb (A5470). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	MAGE-1/MAGE1A Rabbit pAb
Catalog No.	A5470
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human MAGE-1/MAGE1A (NP_004979.3).
Sequence	MSLEQRSLHCKPEEALEAQQEALGLVCVQAATSSSSPLVLGTLEEVPTAGSTDPPQSPQGASAFPTTINFTRQRQPSEGSSSREEEGPSTSCILESLFRAVITKKVADLVGFLLLKYRAREPVTKAEMLESVIKNYKHCFPEIFGKASESLQLVFGIDVKEADPTGHSYVLVTCLGLSYDGLLGDNQIMPKTGFLIIVLVMIAMEGGHAPEEEIWEELSVMEVYDGREHSAYGEPRKLLTQDLVQEKYLEYRQVPDSDPARYEFLWGPRALAETSYVKVLEYVIKVSARVRFFFPSLREAALREEEEGV
Gene ID
Swiss Prot
Synonyms	CT1.1; MAGE1; MAGE-1/MAGE1A
Calculated MW	34kDa
Observed MW	45kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	22Rv1
Cellular location	Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Research Area

MAGE-1/MAGE1A Rabbit pAb images

Western blot - MAGE-1/MAGE1A Rabbit pAb (A5470)

Immunohistochemistry - MAGE-1/MAGE1A Rabbit pAb (A5470)

Immunofluorescence - MAGE-1/MAGE1A Rabbit pAb (A5470)

Immunofluorescence analysis of HeLa cells using MAGE-1/MAGE1A Rabbit pAb (A5470). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.