제품 > 항체 > 다중클론항체(pAb)

MAN1A1 Rabbit pAb (A10830)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates using MAN1A1 Rabbit pAb (A10830) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product name	MAN1A1 Rabbit pAb
Catalog No.	A10830
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a class I mammalian Golgi 1, 2-mannosidase which is a type II transmembrane protein. This protein catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 70-190 of human MAN1A1 (NP_005898.2).
Sequence	SGVLFHSSPALQPAADHKPGPGARAEDAAEGRARRREEGAPGDPEAALEDNLARIRENHERALREAKETLQKLPEEIQRDILLEKKKVAQDQLRDKAPFRGLPPVDFVPPIGVESREPADA
Gene ID
Swiss Prot
Synonyms	MAN9; HUMM3; HUMM9; MAN1A1
Calculated MW	73kDa
Observed MW	73kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T, LO2, Raji, A-549
Cellular location	Golgi apparatus membrane, Single-pass type II membrane protein