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MDM2 Rabbit mAb (A23388)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - MDM2 Rabbit mAb (A23388)

Western blot analysis of lysates from U2OS cells, using MDM2 Rabbit mAb (A23388) at 1:2000 dilution. U2OS cells were treated by Nutlin 3a(10μM ) at 37℃ for 24 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameMDM2 Rabbit mAb
Catalog No.A23388
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60296
This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 101-200 of human MDM2 (NP_001354919.1).
SequenceTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALC
Gene ID
Swiss Prot
SynonymsHDMX; LSKB; hdm2; ACTFS; MDM2
Calculated MW11kDa/14kDa/24kDa/30kDa/33kDa/35kDa/48kDa/49kDa/55kDa
Observed MW90kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU2OS treated by Nutlin 3a
Cellular locationCytoplasm, Nucleus, nucleolus, nucleoplasm.
Customer validation

WB(Homo sapiens,Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - MDM2 Rabbit mAb (A23388)}

Western blot - MDM2 Rabbit mAb (A23388)

Western blot analysis of lysates from U2OS cells, using MDM2 Rabbit mAb (A23388) at 1:2000 dilution. U2OS cells were treated by Nutlin 3a(10μM ) at 37℃ for 24 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (5)

Secondary Antibodies (25)