Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of extracts of various cell lines, using MELK Rabbit mAb (A3530) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min.
| Product name | MELK Rabbit mAb |
|---|---|
| Catalog No. | A3530 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC2032 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MELK (NP_055606.1). |
|---|---|
| Sequence | MKDYDELLKYYELHETIGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNLRHQHICQLYHVLETANKIFMVLEYCPGGELFDYIIS |
| Gene ID | |
| Swiss Prot | |
| Synonyms | HPK38 |
| Calculated MW | 75kDa |
| Observed MW | 75kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | 293T, Jurkat, K-562, Mouse testis, Rat testis |
| Cellular location | Cell membrane, Peripheral membrane protein |
| Customer validation | WB(Homo sapiens) IHC(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A3530? Please let us know so that we can cite the reference in this datasheet.
