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MGEA5 Rabbit pAb (A9033)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MGEA5 Rabbit pAb (A9033)

Western blot analysis of various lysates, using MGEA5 Rabbit pAb (A9033) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - MGEA5 Rabbit pAb (A9033)

Immunohistochemistry analysis of paraffin-embedded Human kidney using MGEA5 Rabbit pAb (A9033) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MGEA5 Rabbit pAb (A9033)

Immunohistochemistry analysis of paraffin-embedded Rat brain using MGEA5 Rabbit pAb (A9033) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameMGEA5 Rabbit pAb
Catalog No.A9033
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1).
SequenceYQEPIMSQGAALSGEPTTLTKEEEKKQPDEEPMDMVVEKQEETDHKNDNQILSEIVEAKMAEELKPMDTDKESIAESKSPEMSMQEDCISDIAPMQTDEQTNKEQFVPGPNEKPLYTAEPVTLEDLQLLADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCGLVMGMFTRLSNCAN
Gene ID
Swiss Prot
SynonymsMEA5; MGEA5; NCOAT
Calculated MW103kDa
Observed MW130kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, HeLa
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - MGEA5 Rabbit pAb (A9033)}

    Western blot - MGEA5 Rabbit pAb (A9033)

    Western blot analysis of various lysates, using MGEA5 Rabbit pAb (A9033) at 1:600 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - MGEA5 Rabbit pAb (A9033)}

    Immunohistochemistry - MGEA5 Rabbit pAb (A9033)

    Immunohistochemistry analysis of paraffin-embedded Human kidney using MGEA5 Rabbit pAb (A9033) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - MGEA5 Rabbit pAb (A9033)}

    Immunohistochemistry - MGEA5 Rabbit pAb (A9033)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using MGEA5 Rabbit pAb (A9033) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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