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MGLL Rabbit PolymAb® (A23306PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MGLL Rabbit PolymAb® (A23306PM)

Western blot analysis of various lysates, using MGLL Rabbit PolymAb® (A23306PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - MGLL Rabbit PolymAb® (A23306PM)

Immunohistochemistry analysis of paraffin-embedded Human liver using MGLL Rabbit PolymAb® (A23306PM) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MGLL Rabbit PolymAb® (A23306PM)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using MGLL Rabbit PolymAb® (A23306PM) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MGLL Rabbit PolymAb® (A23306PM)

Immunofluorescence analysis of paraffin-embedded rat kidney using MGLL Rabbit PolymAb® (A23306PM) at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Overview

Product nameMGLL Rabbit PolymAb®
Catalog No.A23306PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-303 of human MGLL. (NP_001003794.1).
SequenceMPEESSPRRTPQSIPYQDLPHLVNADGQYLFCRYWKPTGTPKALIFVSHGAGEHSGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTATAGTASPP
Gene ID
Swiss Prot
SynonymsMGL; HUK5; MAGL; HU-K5
Calculated MW33kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, Mouse brain, Rat testis
Cellular locationCytoplasm, Membrane, Peripheral membrane protein, cytosol.
ABclonal:Western blot - MGLL Rabbit PolymAb® (A23306PM)}

Western blot - MGLL Rabbit PolymAb® (A23306PM)

Western blot analysis of various lysates, using MGLL Rabbit PolymAb® (A23306PM) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - MGLL Rabbit PolymAb® (A23306PM)}

Immunohistochemistry - MGLL Rabbit PolymAb® (A23306PM)

Immunohistochemistry analysis of paraffin-embedded Human liver using MGLL Rabbit PolymAb® (A23306PM) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MGLL Rabbit PolymAb® (A23306PM)}

Immunohistochemistry - MGLL Rabbit PolymAb® (A23306PM)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using MGLL Rabbit PolymAb® (A23306PM) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - MGLL Rabbit PolymAb® (A23306PM)}

Immunofluorescence - MGLL Rabbit PolymAb® (A23306PM)

Immunofluorescence analysis of paraffin-embedded rat kidney using MGLL Rabbit PolymAb® (A23306PM) at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)