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MGLL Rabbit mAb (A23082)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MGLL Rabbit mAb (A23082)

Western blot analysis of lysates from mouse fat, using MGLL Rabbit mAb (A23082) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - MGLL Rabbit mAb (A23082)

Western blot analysis of lysates from rat heart, using MGLL Rabbit mAb (A23082) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - MGLL Rabbit mAb (A23082)

Immunohistochemistry analysis of MGLL in paraffin-embedded rat heart tissue using MGLL Rabbit mAb (A23082) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MGLL Rabbit mAb (A23082)

Immunohistochemistry analysis of MGLL in paraffin-embedded mouse fat tissue using MGLL Rabbit mAb (A23082) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MGLL Rabbit mAb (A23082)

Immunofluorescence analysis of NIH/3T3 cells using MGLL Rabbit mAb (A23082) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - MGLL Rabbit mAb (A23082)

Immunofluorescence analysis of paraffin-embedded rat kidney using MGLL Rabbit mAb (A23082) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMGLL Rabbit mAb
Catalog No.A23082
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58998
This gene encodes a serine hydrolase of the AB hydrolase superfamily that catalyzes the conversion of monoacylglycerides to free fatty acids and glycerol. The encoded protein plays a critical role in several physiological processes including pain and nociperception through hydrolysis of the endocannabinoid 2-arachidonoylglycerol. Expression of this gene may play a role in cancer tumorigenesis and metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MGLL
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsMGL; HUK5; MAGL; HU-K5; MGLL
Calculated MW33kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse fat, Rat heart
Cellular locationCytoplasm, Membrane, Peripheral membrane protein, cytosol

Documents

Certificate of Compliance

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ABclonal:Western blot - MGLL Rabbit mAb (A23082)}

Western blot - MGLL Rabbit mAb (A23082)

Western blot analysis of lysates from mouse fat, using MGLL Rabbit mAb (A23082) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - MGLL Rabbit mAb (A23082)}

Western blot - MGLL Rabbit mAb (A23082)

Western blot analysis of lysates from rat heart, using MGLL Rabbit mAb (A23082) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - MGLL Rabbit mAb (A23082)}

Immunohistochemistry - MGLL Rabbit mAb (A23082)

Immunohistochemistry analysis of MGLL in paraffin-embedded rat heart tissue using MGLL Rabbit mAb (A23082) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MGLL Rabbit mAb (A23082)}

Immunohistochemistry - MGLL Rabbit mAb (A23082)

Immunohistochemistry analysis of MGLL in paraffin-embedded mouse fat tissue using MGLL Rabbit mAb (A23082) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - MGLL Rabbit mAb (A23082)}

Immunofluorescence - MGLL Rabbit mAb (A23082)

Immunofluorescence analysis of NIH/3T3 cells using MGLL Rabbit mAb (A23082) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - MGLL Rabbit mAb (A23082)}

Immunofluorescence - MGLL Rabbit mAb (A23082)

Immunofluorescence analysis of paraffin-embedded rat kidney using MGLL Rabbit mAb (A23082) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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