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MIB1/DIP1 Rabbit pAb (A8588)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MIB1/DIP1 Rabbit pAb (A8588)

Western blot analysis of various lysates using MIB1/DIP1 Rabbit pAb (A8588) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameMIB1/DIP1 Rabbit pAb
Catalog No.A8588
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 560-860 of human MIB1/DIP1 (NP_065825.1).
SequenceDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPNLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSR
Gene ID
Swiss Prot
SynonymsMIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2; MIB1/DIP1
Calculated MW110kDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, A-549, HepG2, Mouse stomach, Mouse lung, Mouse kidney , Rat testis
Cellular locationCell membrane, Cytoplasm, centriolar satellite, centrosome, cytoskeleton, microtubule organizing center.

Documents

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ABclonal:Western blot - MIB1/DIP1 Rabbit pAb (A8588)}

Western blot - MIB1/DIP1 Rabbit pAb (A8588)

Western blot analysis of various lysates using MIB1/DIP1 Rabbit pAb (A8588) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)