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MKNK1 Rabbit pAb (A18429)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MKNK1 Rabbit pAb (A18429)

Western blot analysis of various lysates, using MKNK1 Rabbit pAb (A18429) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - MKNK1 Rabbit pAb (A18429)

Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using MKNK1 Rabbit pAb (A18429) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MKNK1 Rabbit pAb (A18429)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MKNK1 Rabbit pAb (A18429) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - MKNK1 Rabbit pAb (A18429)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MKNK1 Rabbit pAb (A18429) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MKNK1 Rabbit pAb (A18429)

Immunofluorescence analysis of NIH-3T3 cells using MKNK1 Rabbit pAb (A18429) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMKNK1 Rabbit pAb
Catalog No.A18429
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 374-465 of human MKNK1 (NP_003675.2).
SequenceVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
Gene ID
Swiss Prot
SynonymsMNK1; MKNK1
Calculated MW51kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse skeletal muscle, Rat skeletal muscle
Cellular locationCytoplasm, Cytoplasm, Nucleus.

Documents

Certificate of Compliance

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ABclonal:Western blot - MKNK1 Rabbit pAb (A18429)}

Western blot - MKNK1 Rabbit pAb (A18429)

Western blot analysis of various lysates, using MKNK1 Rabbit pAb (A18429) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - MKNK1 Rabbit pAb (A18429)}

Immunohistochemistry - MKNK1 Rabbit pAb (A18429)

Immunohistochemistry analysis of paraffin-embedded Rat fallopian tube using MKNK1 Rabbit pAb (A18429) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MKNK1 Rabbit pAb (A18429)}

Immunohistochemistry - MKNK1 Rabbit pAb (A18429)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MKNK1 Rabbit pAb (A18429) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - MKNK1 Rabbit pAb (A18429)}

Immunohistochemistry - MKNK1 Rabbit pAb (A18429)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using MKNK1 Rabbit pAb (A18429) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - MKNK1 Rabbit pAb (A18429)}

Immunofluorescence - MKNK1 Rabbit pAb (A18429)

Immunofluorescence analysis of NIH-3T3 cells using MKNK1 Rabbit pAb (A18429) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)