제품 > 항체 > 다중클론항체(pAb)

MMP9 Rabbit pAb (A11147)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using MMP9 Rabbit pAb (A11147) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - MMP9 Rabbit pAb (A11147)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MMP9 Rabbit pAb (A11147) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using MMP9 Rabbit pAb (A11147) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	MMP9 Rabbit pAb
Catalog No.	A11147
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 601-700 of human MMP9 (NP_004985.2).
Sequence	LDKLGLGADVAQVTGALRSGRGKMLLFSGRRLWRFDVKAQMVDPRSASEVDRMFPGVPLDTHDVFQYREKAYFCQDRFYWRVSSRSELNQVDQVGYVTYD
Gene ID
Swiss Prot
Synonyms	GELB; CLG4B; MMP-9; MANDP2; MMP9
Calculated MW	78kDa
Observed MW	92kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Mouse spleen, Rat kidney
Cellular location	Secreted, extracellular matrix, extracellular space.
Customer validation	WB(Sanghuangporus vaninii, Rattus norvegicus, Homo sapiens, Mus musculus, Other) IHC(Mus musculus) IHC(Rattus norvegicus)

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Research Area

MMP9 Rabbit pAb images

Western blot - MMP9 Rabbit pAb (A11147)

Immunohistochemistry - MMP9 Rabbit pAb (A11147)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for MMP9 Rabbit pAb (A11147)

Product:

MMP9 Rabbit pAb

Journal:

Int J Biol Macromol

Application:

IF:

7.7

Species:

Sanghuangporus vaninii

PMID:

32629056

Title:

Characterization of a polysaccharide from Sanghuangporus vaninii and its antitumor regulation via activation of the p53 signaling pathway in breast cancer MCF-7 …

References for MMP9 Rabbit pAb (A11147)

Product:

MMP9 Rabbit pAb

Journal:

J Vasc Res

Application:

IF:

1.8

Species:

Rattus norvegicus

PMID:

33601368

Title:

Metformin Inhibits Abdominal Aortic Aneurysm Formation through the Activation of the AMPK/mTOR Signaling Pathway

References for MMP9 Rabbit pAb (A11147)

Product:

MMP9 Rabbit pAb

Journal:

Cancer science

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

37519194

Title:

Isobutyric acid promotes colorectal cancer metastasis through activating RACK1

References for MMP9 Rabbit pAb (A11147)

Product:

MMP9 Rabbit pAb

Journal:

eLife

Application:

IF:

6.4

Species:

Mus musculus

PMID:

37929702

Title:

GLI1 facilitates collagen-induced arthritis in mice by collaborative regulation of DNA methyltransferases - PMC

References for MMP9 Rabbit pAb (A11147)

Product:

MMP9 Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Mus musculus

PMID:

38173486

Title:

An exploration of the effect of Chinese herbal compound on the occurrence and development of large intestine cancer and intestinal flora - PMC

References for MMP9 Rabbit pAb (A11147)

Product:

MMP9 Rabbit pAb

Journal:

Cardiovascular toxicology

Application:

IF:

3.4

Species:

Mus musculus

PMID:

38310188

Title:

Blockade of ZFX Alleviates Hypoxia-Induced Pulmonary Vascular Remodeling by Regulating the YAP Signaling

References for MMP9 Rabbit pAb (A11147)

Product:

MMP9 Rabbit pAb

Journal:

Heliyon

Application:

IF:

Species:

Homo sapiens

PMID:

38076191

Title:

Inhibition of Pard3 promotes breast cancer metastasis via the USP28 mediated deubiquitination of Snail1 - PMC

References for MMP9 Rabbit pAb (A11147)

Product:

MMP9 Rabbit pAb

Journal:

Cell communication and signaling : CCS

Application:

IF:

7.5

Species:

Mus musculus

PMID:

38439009

Title:

Pan-histone deacetylase inhibitor vorinostat suppresses osteoclastic bone resorption through modulation of RANKL-evoked signaling and ameliorates ovariectomy-induced bone loss - PMC

References for MMP9 Rabbit pAb (A11147)

Product:

MMP9 Rabbit pAb

Journal:

International immunopharmacology

Application:

IHC

IF:

4.8

Species:

Mus musculus

PMID: