제품 > 항체 > 다중클론항체(pAb)

MORF4L2 Rabbit pAb (A7577)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MORF4L2 Rabbit pAb (A7577)

Western blot analysis of extracts of Jurkat cells, using MORF4L2 antibody (A7577) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

ABclonal:Immunofluorescence - MORF4L2 Rabbit pAb (A7577)

Immunofluorescence analysis of A549 cells using MORF4L2 antibody (A7577).

Overview

Product nameMORF4L2 Rabbit pAb
Catalog No.A7577
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in heterochromatin assembly and histone modification. Predicted to act upstream of or within positive regulation of striated muscle cell differentiation and positive regulation of transcription by RNA polymerase II. Located in nucleolus; nucleoplasm; and plasma membrane.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-282 of human MORF4L2 (NP_001135890.1).
SequenceMSSRKQGSQPRGQQSAEEENFKKPTRSNMQRSKMRGASSGKKTAGPQQKNLEPALPGRWGGRSAENPPSGSVRKTRKNKQKTPGNGDGGSTSEAPQPPRKKRARADPTVESEEAFKNRMEVKVKIPEELKPWLVEDWDLVTRQKQLFQLPAKKNVDAILEEYANCKKSQGNVDNKEYAVNEVVAGIKEYFNVMLGTQLLYKFERPQYAEILLAHPDAPMSQVYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLGYLHDFLKYLAKNSASLFTASDYKVASAE
Gene ID
Swiss Prot
SynonymsMRGX; MORFL2
Calculated MW32kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesJurkat
Cellular locationNucleus

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ABclonal:Western blot - MORF4L2 Rabbit pAb (A7577)}

Western blot - MORF4L2 Rabbit pAb (A7577)

Western blot analysis of extracts of Jurkat cells, using MORF4L2 antibody (A7577) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.
ABclonal:Immunofluorescence - MORF4L2 Rabbit pAb (A7577)}

Immunofluorescence - MORF4L2 Rabbit pAb (A7577)

Immunofluorescence analysis of A549 cells using MORF4L2 antibody (A7577).

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)