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MS4A14 Rabbit mAb (A20965)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - MS4A14 Rabbit mAb (A20965)

Western blot analysis of various lysates using MS4A14 Rabbit mAb (A20965) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameMS4A14 Rabbit mAb
Catalog No.A20965
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2954
Predicted to be integral component of membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 150-280 of human MS4A14 (Q96JA4).
SequenceFSRTLFIVLFFLPSDVTQNSEQPAPEENDQLQFVLQEEFSSDDSTTNAQSVIFGGYAFFKLTLSRSPLVSQPGNKGREFVPDEQKQSILPSPKFSEEEIEPLPPTLEKKPSENMSIQLDSTFKQMKDEDLQ
Gene ID
Swiss Prot
SynonymsMS4A16; NYD-SP21; MS4A14
Calculated MW77kDa
Observed MW76kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat testis
Cellular locationPlasma membrane.

Documents

Certificate of Compliance

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    ABclonal:Western blot - MS4A14 Rabbit mAb (A20965)}

    Western blot - MS4A14 Rabbit mAb (A20965)

    Western blot analysis of various lysates using MS4A14 Rabbit mAb (A20965) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)