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MUTA Rabbit mAb (A9339)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - MUTA Rabbit mAb (A9339)

Western blot analysis of various lysates, using (A9339) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - MUTA Rabbit mAb (A9339)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MUTA Rabbit mAb (A9339) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - MUTA Rabbit mAb (A9339)

Immunohistochemistry analysis of paraffin-embedded Human liver using MUTA Rabbit mAb (A9339) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - MUTA Rabbit mAb (A9339)

Immunofluorescence analysis of HepG2 cells using MUTA Rabbit mAb (A9339) at dilution of 1:150. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMUTA Rabbit mAb
Catalog No.A9339
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2733
This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 500-600 of human MUTA (P22033).
SequenceVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSA
Gene ID
Swiss Prot
SynonymsMCM; MUT; MUTA
Calculated MW83kDa
Observed MW83kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2
Cellular locationMitochondrion matrix

Documents

Certificate of Compliance

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ABclonal:Western blot - MUTA Rabbit mAb (A9339)}

Western blot - MUTA Rabbit mAb (A9339)

Western blot analysis of various lysates, using (A9339) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - MUTA Rabbit mAb (A9339)}

Immunohistochemistry - MUTA Rabbit mAb (A9339)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using MUTA Rabbit mAb (A9339) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - MUTA Rabbit mAb (A9339)}

Immunohistochemistry - MUTA Rabbit mAb (A9339)

Immunohistochemistry analysis of paraffin-embedded Human liver using MUTA Rabbit mAb (A9339) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - MUTA Rabbit mAb (A9339)}

Immunofluorescence - MUTA Rabbit mAb (A9339)

Immunofluorescence analysis of HepG2 cells using MUTA Rabbit mAb (A9339) at dilution of 1:150. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

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