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MYH7/β-MHC Rabbit pAb (A7564)

Publications (24) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MYH7/β-MHC Rabbit pAb (A7564)

Western blot analysis of various lysates using MYH7/β-MHC Rabbit pAb (A7564) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - MYH7/β-MHC Rabbit pAb (A7564)

Immunofluorescence analysis of RD cells using MYH7/β-MHC Rabbit pAb (A7564) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameMYH7/β-MHC Rabbit pAb
Catalog No.A7564
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing distal myopathy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1700-1800 of human MYH7/β-MHC (NP_000248.2).
SequenceKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGTKGLNEE
Gene ID
Swiss Prot
SynonymsCMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB; CMYP7A; CMYP7B; MYH7/β-MHC
Calculated MW223kDa
Observed MW250kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse heart, Rat heart
Cellular locationCytoplasm, myofibril.
Customer validation

WB(Sus scrofa, Mus musculus, Capra hircus, Homo sapiens, Mus musculus, Rattus norvegicus)

IF(Mus musculus, Homo sapiens)

IF(Capra hircus, Homo sapiens)

IP(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - MYH7/β-MHC Rabbit pAb (A7564)}

Western blot - MYH7/β-MHC Rabbit pAb (A7564)

Western blot analysis of various lysates using MYH7/β-MHC Rabbit pAb (A7564) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - MYH7/β-MHC Rabbit pAb (A7564)}

Immunofluorescence - MYH7/β-MHC Rabbit pAb (A7564)

Immunofluorescence analysis of RD cells using MYH7/β-MHC Rabbit pAb (A7564) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7564? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)