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MYH7 Rabbit pAb (A24664)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - MYH7 Rabbit pAb (A24664)

Western blot analysis of lysates from wild type (WT) and 293T transfected with MYH7 Rabbit pAb, using MYH7 (A24664) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - MYH7 Rabbit pAb (A24664)

Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using MYH7 Rabbit pAb (A24664) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - MYH7 Rabbit pAb (A24664)

Immunofluorescence analysis of paraffin-embedded Human heart using MYH7 Rabbit pAb (A24664) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - MYH7 Rabbit pAb (A24664)

Immunofluorescence analysis of paraffin-embedded mouse heart using MYH7 Rabbit pAb (A24664) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - MYH7 Rabbit pAb (A24664)

Immunofluorescence analysis of paraffin-embedded rat heart using MYH7 Rabbit pAb (A24664) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameMYH7 Rabbit pAb
Catalog No.A24664
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing distal myopathy.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1240-1340 of human MYH7(NP_000248.2).
SequenceKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDC
Gene ID
Swiss Prot
SynonymsCMH1; MPD1; SPMD; SPMM; CMD1S; MYHCB; CMYP7A; CMYP7B; MYH7
Calculated MW223kDa
Observed MW251kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T transfected with MYH7 (Human)
Cellular locationCytoplasm, myofibril.

Documents

Certificate of Compliance

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ABclonal:Western blot - MYH7 Rabbit pAb (A24664)}

Western blot - MYH7 Rabbit pAb (A24664)

Western blot analysis of lysates from wild type (WT) and 293T transfected with MYH7 Rabbit pAb, using MYH7 (A24664) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - MYH7 Rabbit pAb (A24664)}

Immunohistochemistry - MYH7 Rabbit pAb (A24664)

Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using MYH7 Rabbit pAb (A24664) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - MYH7 Rabbit pAb (A24664)}

Immunofluorescence - MYH7 Rabbit pAb (A24664)

Immunofluorescence analysis of paraffin-embedded Human heart using MYH7 Rabbit pAb (A24664) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - MYH7 Rabbit pAb (A24664)}

Immunofluorescence - MYH7 Rabbit pAb (A24664)

Immunofluorescence analysis of paraffin-embedded mouse heart using MYH7 Rabbit pAb (A24664) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - MYH7 Rabbit pAb (A24664)}

Immunofluorescence - MYH7 Rabbit pAb (A24664)

Immunofluorescence analysis of paraffin-embedded rat heart using MYH7 Rabbit pAb (A24664) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)