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Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Monoamine Oxidase B (MAOB) Rabbit mAb
ABclonal:Western blot - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Western blot analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human colon carcinoma tissue using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human kidney tissue using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human liver tissue using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human tonsil tissue using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameMonoamine Oxidase B (MAOB) Rabbit mAb
Catalog No.A11597
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0641
The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Monoamine Oxidase B (MAOB) (P27338).
SequenceMSNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDRVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFR
Gene ID
Swiss Prot
SynonymsMAOB; monoamine oxidase B
Calculated MW59kDa
Observed MW59kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Hep G2
Cellular locationCytoplasmic side, Mitochondrion outer membrane, Single-pass type IV membrane protein

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ABclonal:Western blot - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)}

Western blot - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Western blot analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)}

Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human colon carcinoma tissue using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)}

Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human kidney tissue using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)}

Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human liver tissue using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)}

Immunohistochemistry - Monoamine Oxidase B (MAOB) Rabbit mAb (A11597)

Immunohistochemistry analysis of Monoamine Oxidase B (MAOB) in paraffin-embedded human tonsil tissue using Monoamine Oxidase B (MAOB) Rabbit mAb (A11597) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)