제품 > 항체 > 단일클론항체(mAb)

NCOA4 Rabbit mAb (A25307)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using NCOA4 Rabbit mAb (A25307) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Review

Overview

Product name	NCOA4 Rabbit mAb
Catalog No.	A25307
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC3242

Background

This gene encodes an androgen receptor coactivator. The encoded protein interacts with the androgen receptor in a ligand-dependent manner to enhance its transcriptional activity. Chromosomal translocations between this gene and the ret tyrosine kinase gene, also located on chromosome 10, have been associated with papillary thyroid carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes are present on chromosomes 4, 5, 10, and 14.

Immunogen information

Immunogen	Synthetic peptide
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	RFG; ELE1; PTC3; ARA70; NCOA4
Calculated MW	70kDa
Observed MW	80kDa/75KD，76KD

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Hep G2, NIH/3T3, Mouse testis, Hep G2
Cellular location	nucleus.
Customer validation	(CT26 cells) WB(Mus musculus)