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NDUFS3 Rabbit pAb (A3983)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - NDUFS3 Rabbit pAb (A3983)

Western blot analysis of extracts of THP-1 cells, using NDUFS3 antibody (A3983).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Western blot - NDUFS3 Rabbit pAb (A3983)

Western blot analysis of extracts of various cell lines, using NDUFS3 Rabbit pAb antibody (A3983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameNDUFS3 Rabbit pAb
Catalog No.A3983
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).
SequenceESAGADTRPTVRPRNDVAHKQLSAFGEYVAEILPKYVQQVQVSCFNELEVCIHPDGVIPVLTFLRDHTNAQFKSLVDLTAVDVPTRQNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESAVSVFKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK
Gene ID
Swiss Prot
SynonymsCI-30; MC1DN8
Calculated MW30kDa
Observed MW26kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesTHP-1
Cellular locationMitochondrion inner membrane

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ABclonal:Western blot - NDUFS3 Rabbit pAb (A3983)}

Western blot - NDUFS3 Rabbit pAb (A3983)

Western blot analysis of extracts of THP-1 cells, using NDUFS3 antibody (A3983).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Western blot - NDUFS3 Rabbit pAb (A3983)}

Western blot - NDUFS3 Rabbit pAb (A3983)

Western blot analysis of extracts of various cell lines, using NDUFS3 Rabbit pAb antibody (A3983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)