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NF-κB2 Rabbit pAb (A3108)

Publications (13) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NF-κB2 Rabbit pAb (A3108)

Western blot analysis of various lysates using NF-κB2 Rabbit pAb (A3108) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - NF-κB2 Rabbit pAb (A3108)

Immunohistochemistry analysis of paraffin-embedded Rat brain using NF-κB2 Rabbit pAb (A3108) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - NF-κB2 Rabbit pAb (A3108)

Immunohistochemistry analysis of paraffin-embedded Human breast using NF-κB2 Rabbit pAb (A3108) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - NF-κB2 Rabbit pAb (A3108)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using NF-κB2 Rabbit pAb (A3108) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - NF-κB2 Rabbit pAb (A3108)

Immunofluorescence analysis of U2OS cells using NF-κB2 Rabbit pAb (A3108) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNF-κB2 Rabbit pAb
Catalog No.A3108
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 690-899 of human NF-κB2 (NP_002493.3).
SequenceAGADIHAENEEPLCPLPSPPTSDSDSDSEGPEKDTRSSFRGHTPLDLTCSTKVKTLLLNAAQNTMEPPLTPPSPAGPGLSLGDTALQNLEQLLDGPEAQGSWAELAERLGLRSLVDTYRQTTSPSGSLLRSYELAGGDLAGLLEALSDMGLEEGVRLLRGPETRDKLPSTEVKEDSAYGSQSVEQEAEKLGPPPEPPGGLCHGHPQPQVH
Gene ID
Swiss Prot
Synonymsp52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100; NF-κB2
Calculated MW97kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Jurkat, H460, 293T, Mouse thymus
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Capra hircus, Mus musculus, Rattus norvegicus)

IHC(Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - NF-κB2 Rabbit pAb (A3108)}

Western blot - NF-κB2 Rabbit pAb (A3108)

Western blot analysis of various lysates using NF-κB2 Rabbit pAb (A3108) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - NF-κB2 Rabbit pAb (A3108)}

Immunohistochemistry - NF-κB2 Rabbit pAb (A3108)

Immunohistochemistry analysis of paraffin-embedded Rat brain using NF-κB2 Rabbit pAb (A3108) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - NF-κB2 Rabbit pAb (A3108)}

Immunohistochemistry - NF-κB2 Rabbit pAb (A3108)

Immunohistochemistry analysis of paraffin-embedded Human breast using NF-κB2 Rabbit pAb (A3108) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - NF-κB2 Rabbit pAb (A3108)}

Immunohistochemistry - NF-κB2 Rabbit pAb (A3108)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using NF-κB2 Rabbit pAb (A3108) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - NF-κB2 Rabbit pAb (A3108)}

Immunofluorescence - NF-κB2 Rabbit pAb (A3108)

Immunofluorescence analysis of U2OS cells using NF-κB2 Rabbit pAb (A3108) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3108? Please let us know so that we can cite the reference in this datasheet.

항체 (6)

Secondary Antibodies (26)