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Phospho-NF-κB2-S866 Rabbit mAb (AP1367)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - Phospho-NF-κB2-S866 Rabbit mAb (AP1367)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-κB2-S866 Rabbit mAb (AP1367) at 1:500 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product namePhospho-NF-κB2-S866 Rabbit mAb
Catalog No.AP1367
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56344
This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic phosphorylated peptide around S866 of human Phospho-NF-κB2-S866 (NP_001070962.1).
SequenceEDSAY
Gene ID
Swiss Prot
Synonymsp52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100; Phospho-NF-κB2-S866
Calculated MW97kDa
Observed MW140kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNIH/3T3 treated by TNF-α
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - Phospho-NF-κB2-S866 Rabbit mAb (AP1367)}

Western blot - Phospho-NF-κB2-S866 Rabbit mAb (AP1367)

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NF-κB2-S866 Rabbit mAb (AP1367) at 1:500 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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