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NLRC5 Rabbit pAb (A16710)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - NLRC5 Rabbit pAb (A16710)

Western blot analysis of lysates from mouse lung, using NLRC5 Rabbit pAb (A16710) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5min.

Overview

Product nameNLRC5 Rabbit pAb
Catalog No.A16710
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the caspase recruitment domain-containing NLR family. This gene plays a role in cytokine response and antiviral immunity through its inhibition of NF-kappa-B activation and negative regulation of type I interferon signaling pathways.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NLRC5 (NP_115582.4).
SequenceMDPVGLQLGNKNLWSCLVRLLTKDPEWLNAKMKFFLPNTDLDSRNETLDPEQRVILQLNKLHVQGSDTWQSFIHCVCMQLEVPLDLEVLLLSTFGYDDGFTSQLGAEGKSQPESQLHHGLKRPHQSCGSSPRRKQCKKQQLELAKKYLQLLRTSAQQRYRSQIPGSGQPHAFHQVYVPPILRRATASLDTPEGAIMGDVKVEDGADVSISDLFNTRVNKGPRVTVLLGKAGMGKTTLAHR
Gene ID
Swiss Prot
SynonymsNOD4; NOD27; CLR16.1; NLRC5
Calculated MW205kDa
Observed MW202kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesmouse lung
Cellular locationcentrosome, cytoplasm, cytosol, nucleus.
    ABclonal:Western blot - NLRC5 Rabbit pAb (A16710)}

    Western blot - NLRC5 Rabbit pAb (A16710)

    Western blot analysis of lysates from mouse lung, using NLRC5 Rabbit pAb (A16710) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5min.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)