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NNMT Rabbit pAb (A14030)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NNMT Rabbit pAb (A14030)

Western blot analysis of extracts of various cell lines, using NNMT antibody (A14030) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - NNMT Rabbit pAb (A14030)

Immunofluorescence analysis of mouse liver cells using NNMT Rabbit pAb (A14030) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameNNMT Rabbit pAb
Catalog No.A14030
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human NNMT (NP_006160.1).
SequenceMESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKNLFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEPEAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCVLSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGREAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL
Gene ID
Swiss Prot
SynonymsNNMT
Calculated MW30kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2, SKOV3, Mouse liver, Rat lung
Cellular locationCytoplasm
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - NNMT Rabbit pAb (A14030)}

Western blot - NNMT Rabbit pAb (A14030)

Western blot analysis of extracts of various cell lines, using NNMT antibody (A14030) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - NNMT Rabbit pAb (A14030)}

Immunofluorescence - NNMT Rabbit pAb (A14030)

Immunofluorescence analysis of mouse liver cells using NNMT Rabbit pAb (A14030) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A14030? Please let us know so that we can cite the reference in this datasheet.

항체 (5)

Secondary Antibodies (26)