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NPM1 Rabbit pAb (A17983)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NPM1 Rabbit pAb (A17983)

Western blot analysis of various lysates using NPM1 Rabbit pAb (A17983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - NPM1 Rabbit pAb (A17983)

Western blot analysis of various lysates using NPM1 Rabbit pAb (A17983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - NPM1 Rabbit pAb (A17983)

Immunohistochemistry analysis of paraffin-embedded human liver using NPM1 Rabbit pAb (A17983) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NPM1 Rabbit pAb (A17983)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using NPM1 Rabbit pAb (A17983) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NPM1 Rabbit pAb (A17983)

Immunohistochemistry analysis of paraffin-embedded rat testis using NPM1 Rabbit pAb (A17983) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NPM1 Rabbit pAb (A17983)

Immunofluorescence analysis of U2OS cells using NPM1 Rabbit pAb (A17983) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNPM1 Rabbit pAb
Catalog No.A17983
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 9-122 of human NPM1 (NP_002511.1).
SequenceMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEED
Gene ID
Swiss Prot
SynonymsB23; NPM; NPM1
Calculated MW33kDa
Observed MW38kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, Jurkat, HeLa, NIH/3T3, Mouse thymus, Mouse spleen
Cellular locationCytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus, nucleoplasm
Customer validation

WB(Homo sapiens)

IP(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - NPM1 Rabbit pAb (A17983)}

Western blot - NPM1 Rabbit pAb (A17983)

Western blot analysis of various lysates using NPM1 Rabbit pAb (A17983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - NPM1 Rabbit pAb (A17983)}

Western blot - NPM1 Rabbit pAb (A17983)

Western blot analysis of various lysates using NPM1 Rabbit pAb (A17983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - NPM1 Rabbit pAb (A17983)}

Immunohistochemistry - NPM1 Rabbit pAb (A17983)

Immunohistochemistry analysis of paraffin-embedded human liver using NPM1 Rabbit pAb (A17983) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NPM1 Rabbit pAb (A17983)}

Immunohistochemistry - NPM1 Rabbit pAb (A17983)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using NPM1 Rabbit pAb (A17983) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - NPM1 Rabbit pAb (A17983)}

Immunohistochemistry - NPM1 Rabbit pAb (A17983)

Immunohistochemistry analysis of paraffin-embedded rat testis using NPM1 Rabbit pAb (A17983) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NPM1 Rabbit pAb (A17983)}

Immunofluorescence - NPM1 Rabbit pAb (A17983)

Immunofluorescence analysis of U2OS cells using NPM1 Rabbit pAb (A17983) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A17983? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)