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Phospho-NPM1-T199 Rabbit pAb (AP0836)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-NPM1-T199 Rabbit pAb (AP0836)

Western blot analysis of various lysates, using Phospho-NPM1-T199 Rabbit pAb (AP0836) at 1:900 dilution. HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product namePhospho-NPM1-T199 Rabbit pAb
Catalog No.AP0836
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified.
ImmunogenA synthetic phosphorylated peptide around T199 of human NPM1 (NP_001032827.1).
SequenceRDTPA
Gene ID
Swiss Prot
SynonymsB23; NPM; Phospho-NPM1-T199
Calculated MW33kDa
Observed MW38kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa treated by nocodazole
Cellular locationCytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus, nucleoplasm.

Documents

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ABclonal:Western blot - Phospho-NPM1-T199 Rabbit pAb (AP0836)}

Western blot - Phospho-NPM1-T199 Rabbit pAb (AP0836)

Western blot analysis of various lysates, using Phospho-NPM1-T199 Rabbit pAb (AP0836) at 1:900 dilution. HeLa cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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