제품 > 항체 > 다중클론항체(pAb)

NPY Rabbit pAb (A3178)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NPY Rabbit pAb (A3178)

Western blot analysis of lysates from SH-SY5Y cells, using NPY Rabbit pAb (A3178) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - NPY Rabbit pAb (A3178)

Immunohistochemistry analysis of paraffin-embedded Rat brain using NPY Rabbit pAb (A3178) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - NPY Rabbit pAb (A3178)

Immunofluorescence analysis of SH-SY5Y cells using NPY Rabbit pAb (A3178) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNPY Rabbit pAb
Catalog No.A3178
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 29-97 of NPY (NP_000896.1).
SequenceYPSKPDNPGEDAPAEDMARYYSALRHYINLITRQRYGKRSSPETLISDLLMRESTENVPRTRLEDPAMW
Gene ID
Swiss Prot
SynonymsPYY4; NPY
Calculated MW11kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y
Cellular locationSecreted.
Customer validation

WB(Sus scrofa, Rattus norvegicus, Mus musculus )

IHC(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - NPY Rabbit pAb (A3178)}

Western blot - NPY Rabbit pAb (A3178)

Western blot analysis of lysates from SH-SY5Y cells, using NPY Rabbit pAb (A3178) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - NPY Rabbit pAb (A3178)}

Immunohistochemistry - NPY Rabbit pAb (A3178)

Immunohistochemistry analysis of paraffin-embedded Rat brain using NPY Rabbit pAb (A3178) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - NPY Rabbit pAb (A3178)}

Immunofluorescence - NPY Rabbit pAb (A3178)

Immunofluorescence analysis of SH-SY5Y cells using NPY Rabbit pAb (A3178) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A3178? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)