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NTF3 Rabbit pAb (A3179)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - NTF3 Rabbit pAb (A3179)

Western blot analysis of lysates from mouse heart, using NTF3 Rabbit pAb (A3179) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - NTF3 Rabbit pAb (A3179)

Immunohistochemistry analysis of paraffin-embedded human esophagus using NTF3 Rabbit pAb (A3179) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - NTF3 Rabbit pAb (A3179)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using NTF3 Rabbit pAb (A3179) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameNTF3 Rabbit pAb
Catalog No.A3179
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 19-257 of human NTF3 (NP_002518.1).
SequenceNNMDQRSLPEDSLNSLIIKLIQADILKNKLSKQMVDVKENYQSTLPKAEAPREPERGGPAKSAFQPVIAMDTELLRQQRRYNSPRVLLSDSTPLEPPPLYLMEDYVGSPVVANRTSRRKRYAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT
Gene ID
Swiss Prot
SynonymsNT3; HDNF; NGF2; NT-3; NGF-2; NTF3
Calculated MW29kDa
Observed MW29kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:50 - 1:200
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse heart
Cellular locationSecreted

Documents

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    ABclonal:Western blot - NTF3 Rabbit pAb (A3179)}

    Western blot - NTF3 Rabbit pAb (A3179)

    Western blot analysis of lysates from mouse heart, using NTF3 Rabbit pAb (A3179) at 1:200 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    ABclonal:Immunohistochemistry - NTF3 Rabbit pAb (A3179)}

    Immunohistochemistry - NTF3 Rabbit pAb (A3179)

    Immunohistochemistry analysis of paraffin-embedded human esophagus using NTF3 Rabbit pAb (A3179) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - NTF3 Rabbit pAb (A3179)}

    Immunohistochemistry - NTF3 Rabbit pAb (A3179)

    Immunohistochemistry analysis of paraffin-embedded mouse kidney using NTF3 Rabbit pAb (A3179) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)