제품 > 항체 > 다중클론항체(pAb)

NUP133 Rabbit pAb (A8818)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - NUP133 Rabbit pAb (A8818)

Western blot analysis of extracts of various cell lines, using NUP133 antibody (A8818) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

Overview

Product nameNUP133 Rabbit pAb
Catalog No.A8818
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 730-930 of human NUP133 (NP_060700.2).
SequenceNILKDMLQAASHYRQNRNSLYRREESLEKEPEYVPWTATSGPGGIRTVIIRQHEIVLKVAYPQADSNLRNIVTEQLVALIDCFLDGYVSQLKSVDKSSNRERYDNLEMEYLQKRSDLLSPLLSLGQYLWAASLAEKYCDFDILVQMCEQTDNQSRLQRYMTQFADQNFSDFLFRWYLEKGKRGKLLSQPISQHGQLANFLQ
Gene ID
Swiss Prot
SynonymsGAMOS8; NPHS18; hNUP133
Calculated MW129kDa
Observed MW133kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA375, LO2, HeLa, NCI-H460
Cellular locationChromosome, Nucleus, centromere, kinetochore, nuclear pore complex
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - NUP133 Rabbit pAb (A8818)}

    Western blot - NUP133 Rabbit pAb (A8818)

    Western blot analysis of extracts of various cell lines, using NUP133 antibody (A8818) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A8818? Please let us know so that we can cite the reference in this datasheet.