제품 > 항체 > 단일클론항체(mAb)

Na+/K+-ATPase Rabbit mAb (A11683)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from HeLa cells, using Na+/K+-ATPase Rabbit mAb (A11683) at 1:50000 dilution. Membrane protein extract isolated from HeLa cells.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of various lysates using Na+/K+-ATPase Rabbit mAb (A11683) at 1:50000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Na+/K+-ATPase Rabbit mAb (A11683)

Confocal imaging of human colon using Na+/K+-ATPase Rabbit mAb (A11683, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Review

Overview

Product name	Na+/K+-ATPase Rabbit mAb
Catalog No.	A11683
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0674

Background

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Na+/K+-ATPase (P05023).
Sequence	MGKGVGRDKYEPAAVSEQGDKKGKKGKKDRDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTSARAAEILARDGPNALTPPPTTPEWIKFCRQLFGGF
Gene ID
Swiss Prot
Synonyms	CMT2DD; HOMGSMR2; Na+/K+-ATPase
Calculated MW	113kDa
Observed MW	113kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:50000 - 1:200000 IHC-P 1:400 - 1:4000 IF/ICC 1:100 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	HeLa, 293T, C2C12, C6
Cellular location	Cell membrane, Melanosome, Multi-pass membrane protein.
Customer validation	(Mouse fibroblast) WB(Mus musculus, Scylla, homo sapiens) IF(Homo sapiens)

Documents

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연구단백질 인산화

Na+/K+-ATPase Rabbit mAb images

Western blot - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunofluorescence - Na+/K+-ATPase Rabbit mAb (A11683)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

iScience

Application:

IF:

5.8

Species:

Mus musculus

PMID:

35712077

Title:

Envelope virus-mimetic nanovaccines by hybridizing bioengineered cell membranes with bacterial vesicles

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

Biochimica et biophysica acta. Molecular basis of disease

Application:

IF:

4.2

Species:

Mus musculus

PMID:

36822448

Title:

GLUT4 degradation by GLUTFOURINH® in mice resembles moderate-obese diabetes of human with hyperglycemia and low lipid accumulation

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

Animal nutrition (Zhongguo xu mu shou yi xue hui)

Application:

IF:

6.1

Species:

Scylla

PMID:

37013080

Title:

High dietary lipid level promotes low salinity adaptation in the marine euryhaline crab (Scylla paramamosain)

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

Cell research

Application:

IF:

28.1

Species:

homo sapiens

PMID:

37674011

Title:

Cryo-EM structures of human organic anion transporting polypeptide OATP1B1

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

Autophagy

Application:

IF:

14.6

Species:

Mus musculus

PMID:

38566321

Title:

The matrix protein of lyssavirus hijacks autophagosome for efficient egress by recruiting NEDD4 through its PPxY motif

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

CNS neuroscience & therapeutics

Application:

IF:

5.5

Species:

Mus musculus

PMID:

37248637

Title:

Gm527 deficiency in dentate gyrus improves memory through upregulating dopamine D1 receptor pathway

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

iScience

Application:

IF:

5.8

Species:

Homo sapiens

PMID:

38638566

Title:

Frequent loss of FAM126A expression in colorectal cancer results in selective FAM126B dependency - PMC

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

Neuroscience

Application:

IF:

2.9

Species:

Homo sapiens

PMID:

38964454

Title:

The de novo missense mutation F224S in GABRB2, identified in epileptic encephalopathy and developmental delay, impairs GABAAR function

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

Cell discovery

Application:

IF:

33.5

Species:

Homo sapiens

PMID:

39433541

Title:

NUMB dysfunction defines a novel mechanism underlying hyperuricemia and gout - PMC

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

PloS one

Application:

IF:

2.9

Species:

Mus musculus

PMID:

39637010

Title:

TRPML1 acts as a predisposing factor in lymphedema development by regulating the subcellular localization of aquaporin-3, -5 - PMC

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

Nature communications

Application:

IF:

14.7

Species:

Homo sapiens

PMID:

39747030

Title:

Drug inhibition and substrate transport mechanisms of human VMAT2 - PMC

References for Na+/K+-ATPase Rabbit mAb (A11683)

Product:

Na+/K+-ATPase Rabbit mAb

Journal:

Inflammation research : official journal of the European Histamine Research Society ... [et al.]

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38733398

Title:

Extracellular histones promote TWIK2-dependent potassium efflux and associated NLRP3 activation in alveolar macrophages during sepsis-induced lung injury