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Na+/K+-ATPase Rabbit mAb (A11683)

Publications (9) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Na+/K+-ATPase Rabbit mAb (A11683)

Western blot analysis of lysates from HeLa cells, using Na+/K+-ATPase Rabbit mAb (A11683) at 1:50000 dilution. Membrane protein extract isolated from HeLa cells.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Na+/K+-ATPase Rabbit mAb (A11683)

Western blot analysis of various lysates using Na+/K+-ATPase Rabbit mAb (A11683) at 1:50000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Na+/K+-ATPase Rabbit mAb (A11683)

Confocal imaging of human colon using Na+/K+-ATPase Rabbit mAb (A11683, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Overview

Product nameNa+/K+-ATPase Rabbit mAb
Catalog No.A11683
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0674
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Na+/K+-ATPase (P05023).
SequenceMGKGVGRDKYEPAAVSEQGDKKGKKGKKDRDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTSARAAEILARDGPNALTPPPTTPEWIKFCRQLFGGF
Gene ID
Swiss Prot
SynonymsCMT2DD; HOMGSMR2; Na+/K+-ATPase
Calculated MW113kDa
Observed MW113kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:50000 - 1:200000
  • IHC-P 1:400 - 1:4000
  • IF/ICC 1:100 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, 293T, C2C12, C6
Cellular locationCell membrane, Melanosome, Multi-pass membrane protein.
Customer validation

WB(Mus musculus, Scylla, homo sapiens)

IF(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Na+/K+-ATPase Rabbit mAb (A11683)}

Western blot - Na+/K+-ATPase Rabbit mAb (A11683)

Western blot analysis of lysates from HeLa cells, using Na+/K+-ATPase Rabbit mAb (A11683) at 1:50000 dilution. Membrane protein extract isolated from HeLa cells.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - Na+/K+-ATPase Rabbit mAb (A11683)}

Western blot - Na+/K+-ATPase Rabbit mAb (A11683)

Western blot analysis of various lysates using Na+/K+-ATPase Rabbit mAb (A11683) at 1:50000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)}

Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)}

Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)}

Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)}

Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)}

Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)}

Immunohistochemistry - Na+/K+-ATPase Rabbit mAb (A11683)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Na+/K+-ATPase Rabbit mAb (A11683) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Na+/K+-ATPase Rabbit mAb (A11683)}

Immunofluorescence - Na+/K+-ATPase Rabbit mAb (A11683)

Confocal imaging of human colon using Na+/K+-ATPase Rabbit mAb (A11683, at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A11683? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

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ELISAWB

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ELISAWB

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Secondary Antibodies (26)

HRP-conjugated Goat anti-Rabbit IgG (H+L)
AS014

WBDBIHC-PELISA

HRP-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Goat anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC-conjugated Goat anti-Rabbit IgG (H+L)

Cy3-conjugated Goat anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain
AS061

WBIP

HRP-conjugated Mouse anti-Rabbit IgG Light Chain

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
AS040

IHC-PIF/ICCFC

Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

WBELISACUT&Tag

Goat Anti-Rabbit IgG(H+L)

HRP-conjugated Donkey anti-Rabbit IgG (H+L)
AS038

WBIHC-P

HRP-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

FITC-conjugated Donkey anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC-conjugated Donkey anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

AP-conjugated Goat anti-Rabbit IgG (H+L)
AS009

WB

AP-conjugated Goat anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated Mouse anti-Rabbit IgG (H+L)

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
AS126

IPELISA

HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

PE Mouse anti-Rabbit IgG, light chain specific mAb
AS129

FC

PE Mouse anti-Rabbit IgG, light chain specific mAb

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
AS122

WBELISA

HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)