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Phospho-Na+/K+-ATPase-S16 Rabbit pAb (AP1065)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-Na+/K+-ATPase-S16 Rabbit pAb (AP1065)

Western blot analysis of lysates from 293T cells, using Phospho-Na+/K+-ATPase-S16 Rabbit pAb (AP1065) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product namePhospho-Na+/K+-ATPase-S16 Rabbit pAb
Catalog No.AP1065
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic phosphorylated peptide around S16 of human Na+/K+-ATPase (NP_000692.2).
SequenceAVSEQ
Gene ID
Swiss Prot
SynonymsCMT2DD; HOMGSMR2; Phospho-Na+/K+-ATPase-S16
Calculated MW113kDa
Observed MW112kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T treated by PMA
Cellular locationCell membrane, Melanosome, Multi-pass membrane protein.
Customer validation

WB(Rattus norvegicus)

Documents

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ABclonal:Western blot - Phospho-Na+/K+-ATPase-S16 Rabbit pAb (AP1065)}

Western blot - Phospho-Na+/K+-ATPase-S16 Rabbit pAb (AP1065)

Western blot analysis of lysates from 293T cells, using Phospho-Na+/K+-ATPase-S16 Rabbit pAb (AP1065) at 1:1000 dilution. 293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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