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Na+/K+-ATPase Rabbit pAb (A7878)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Na+/K+-ATPase Rabbit pAb (A7878)

Western blot analysis of various lysates using Na+/K+-ATPase Rabbit pAb (A7878) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit pAb (A7878)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Na+/K+-ATPase Rabbit pAb (A7878) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit pAb (A7878)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Na+/K+-ATPase Rabbit pAb (A7878) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit pAb (A7878)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Na+/K+-ATPase Rabbit pAb (A7878) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Na+/K+-ATPase Rabbit pAb (A7878)

Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using Na+/K+-ATPase Rabbit pAb (A7878) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameNa+/K+-ATPase Rabbit pAb
Catalog No.A7878
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human Na+/K+-ATPase (NP_000692.2).
SequenceMGKGVGRDKYEPAAVSEQGDKKGKKGKKDRDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGLTSARAAEILARDGPNALTPPPTTPEWIKFCRQLFGGF
Gene ID
Swiss Prot
SynonymsCMT2DD; HOMGSMR2; Na+/K+-ATPase
Calculated MW113kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HeLa membrane protein, C2C12, C6
Cellular locationCell membrane, Melanosome, Multi-pass membrane protein.
Customer validation

WB(Mus musculus, Capra hircus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - Na+/K+-ATPase Rabbit pAb (A7878)}

Western blot - Na+/K+-ATPase Rabbit pAb (A7878)

Western blot analysis of various lysates using Na+/K+-ATPase Rabbit pAb (A7878) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit pAb (A7878)}

Immunohistochemistry - Na+/K+-ATPase Rabbit pAb (A7878)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Na+/K+-ATPase Rabbit pAb (A7878) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit pAb (A7878)}

Immunohistochemistry - Na+/K+-ATPase Rabbit pAb (A7878)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Na+/K+-ATPase Rabbit pAb (A7878) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Na+/K+-ATPase Rabbit pAb (A7878)}

Immunohistochemistry - Na+/K+-ATPase Rabbit pAb (A7878)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Na+/K+-ATPase Rabbit pAb (A7878) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Na+/K+-ATPase Rabbit pAb (A7878)}

Immunofluorescence - Na+/K+-ATPase Rabbit pAb (A7878)

Immunofluorescence analysis of paraffin-embedded Mouse colon tissue using Na+/K+-ATPase Rabbit pAb (A7878) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7878? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)