Nanog Rabbit mAb (A25887)

Western blot analysis of lysates from NCCIT cells using Nanog Rabbit mAb (A25887) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from F9 cells, using 5 μg of Nanog Rabbit mAb (A25887) and Rabbit Control IgG (AC005). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.

Flow cytometry: 1X10^6 HeLa cells (negative control, left) and NTERA-2 cells (right) were intracellularly-stained with Nanog Rabbit mAb (A25887, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 NTERA-2 cells were intracellularly-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or Nanog Rabbit mAb (A25887, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and F9 cells (right) were intracellularly-stained with Nanog Rabbit mAb (A25887, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 F9 cells were intracellularly-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or Nanog Rabbit mAb (A25887, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.