Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from THP-1 cells, using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of paraffin-embedded mouse bone marrow using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of paraffin-embedded rat bone marrow using Neutrophil Elastase (ELANE) Rabbit pAb (A13015) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | Neutrophil Elastase (ELANE) Rabbit pAb |
|---|---|
| Catalog No. | A13015 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 30-267 of human Neutrophil Elastase (ELANE) (NP_001963.1). |
|---|---|
| Sequence | IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH |
| Gene ID | |
| Swiss Prot | |
| Synonyms | GE; NE; HLE; HNE; ELA2; SCN1; PMN-E; Neutrophil Elastase (ELANE) |
| Calculated MW | 29kDa |
| Observed MW | 36kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | THP-1 |
| Cellular location | cell surface, cytoplasm, cytosol, extracellular exosome, extracellular region, extracellular space, phagocytic vesicle |
| Customer validation | WB(Rattus norvegicus, Homo sapiens, Mus musculus) IF(Mus musculus, Homo sapiens) IHC(Rattus norvegicus) IHC(Rattus norvegicus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
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