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Nogo Rabbit mAb (A2315)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Nogo Rabbit mAb (A2315)

Western blot analysis of lysates from SH-SY5Y cells, using Nogo Rabbit mAb (A2315) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Nogo Rabbit mAb (A2315)

Immunofluorescence analysis of C6 cells using Nogo Rabbit mAb (A2315) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Nogo Rabbit mAb (A2315)

Immunofluorescence analysis of NIH-3T3 cells using Nogo Rabbit mAb (A2315) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Nogo Rabbit mAb (A2315)

Immunofluorescence analysis of U-2 OS cells using Nogo Rabbit mAb (A2315) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameNogo Rabbit mAb
Catalog No.A2315
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1910
This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 60-160 of human Nogo (NP_065393.1).
SequenceAAGLSAAPVPTAPAAGAPLMDFGNDFVPPAPRGPLPAAPPVAPERQPSWDPSPVSSTVPAPSPLSAAAVSPSKLPEDDEPPARPPPPPPASVSPQAEPVWT
Gene ID
Swiss Prot
SynonymsASY; NSP; NOGO; RTN-X; NSP-CL; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545; Nogo
Calculated MW130kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSH-SY5Y
Cellular locationEndoplasmic reticulum, Glutamatergic synapse, Neuron projection, Nuclear envelope, Plasma membrane, Postsynaptic density
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Nogo Rabbit mAb (A2315)}

Western blot - Nogo Rabbit mAb (A2315)

Western blot analysis of lysates from SH-SY5Y cells, using Nogo Rabbit mAb (A2315) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - Nogo Rabbit mAb (A2315)}

Immunofluorescence - Nogo Rabbit mAb (A2315)

Immunofluorescence analysis of C6 cells using Nogo Rabbit mAb (A2315) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Nogo Rabbit mAb (A2315)}

Immunofluorescence - Nogo Rabbit mAb (A2315)

Immunofluorescence analysis of NIH-3T3 cells using Nogo Rabbit mAb (A2315) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Nogo Rabbit mAb (A2315)}

Immunofluorescence - Nogo Rabbit mAb (A2315)

Immunofluorescence analysis of U-2 OS cells using Nogo Rabbit mAb (A2315) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)