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RTN4 Rabbit pAb (A1752)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - RTN4 Rabbit pAb (A1752)

Western blot analysis of lysates from mouse brain, using RTN4 Rabbit pAb (A1752) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.

ABclonal:Immunofluorescence - RTN4 Rabbit pAb (A1752)

Immunofluorescence analysis of paraffin-embedded rat testis using RTN4 Rabbit pAb (A1752) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RTN4 Rabbit pAb (A1752)

Immunofluorescence analysis of paraffin-embedded rat brain using RTN4 Rabbit pAb (A1752) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RTN4 Rabbit pAb (A1752)

Immunofluorescence analysis of paraffin-embedded mouse testis using RTN4 Rabbit pAb (A1752) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - RTN4 Rabbit pAb (A1752)

Immunofluorescence analysis of paraffin-embedded mouse brain using RTN4 Rabbit pAb (A1752) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameRTN4 Rabbit pAb
Catalog No.A1752
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 200-300 of human RTN4 (NP_065393.1).
SequenceIRSSAENMDLKEQPGNTISAGQEDFPSVLLETAASLPSLSPLSAASFKEHEYLGNLSTVLPTEGTLQENVSEASKEVSEKAKTLLIDRDLTEFSELEYSEM
Gene ID
Swiss Prot
SynonymsASY; NSP; NOGO; RTN-X; NSP-CL; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545; RTN4
Calculated MW130kDa
Observed MW200kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesmouse brain
Cellular locationEndoplasmic reticulum membrane, Multi-pass membrane protein.

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ABclonal:Western blot - RTN4 Rabbit pAb (A1752)}

Western blot - RTN4 Rabbit pAb (A1752)

Western blot analysis of lysates from mouse brain, using RTN4 Rabbit pAb (A1752) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.
ABclonal:Immunofluorescence - RTN4 Rabbit pAb (A1752)}

Immunofluorescence - RTN4 Rabbit pAb (A1752)

Immunofluorescence analysis of paraffin-embedded rat testis using RTN4 Rabbit pAb (A1752) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RTN4 Rabbit pAb (A1752)}

Immunofluorescence - RTN4 Rabbit pAb (A1752)

Immunofluorescence analysis of paraffin-embedded rat brain using RTN4 Rabbit pAb (A1752) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RTN4 Rabbit pAb (A1752)}

Immunofluorescence - RTN4 Rabbit pAb (A1752)

Immunofluorescence analysis of paraffin-embedded mouse testis using RTN4 Rabbit pAb (A1752) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - RTN4 Rabbit pAb (A1752)}

Immunofluorescence - RTN4 Rabbit pAb (A1752)

Immunofluorescence analysis of paraffin-embedded mouse brain using RTN4 Rabbit pAb (A1752) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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