제품 > 항체 > 다중클론항체(pAb)

OXSR1 Rabbit pAb (A15126)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - OXSR1 Rabbit pAb (A15126)

Western blot analysis of various lysates, using OXSR1 Rabbit pAb (A15126) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - OXSR1 Rabbit pAb (A15126)

Immunofluorescence analysis of L929 cells using OXSR1 Rabbit pAb (A15126) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameOXSR1 Rabbit pAb
Catalog No.A15126
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human OXSR1 (NP_005100.1).
SequenceMSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSV
Gene ID
Swiss Prot
SynonymsOSR1; OXSR1
Calculated MW58kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-251MG, 293T, Jurkat
Cellular locationCytoplasm

Documents

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    ABclonal:Western blot - OXSR1 Rabbit pAb (A15126)}

    Western blot - OXSR1 Rabbit pAb (A15126)

    Western blot analysis of various lysates, using OXSR1 Rabbit pAb (A15126) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - OXSR1 Rabbit pAb (A15126)}

    Immunofluorescence - OXSR1 Rabbit pAb (A15126)

    Immunofluorescence analysis of L929 cells using OXSR1 Rabbit pAb (A15126) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)