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Oncostatin M Rabbit pAb (A6163)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Oncostatin M Rabbit pAb (A6163)

Western blot analysis of extracts of various cell lines, using Oncostatin M Antibody (A6163) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunofluorescence - Oncostatin M Rabbit pAb (A6163)

Immunofluorescence analysis of C6 cells using Oncostatin M antibody (A6163) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Oncostatin M Rabbit pAb (A6163)

Immunofluorescence analysis of L-929 cells using Oncostatin M antibody (A6163) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameOncostatin M Rabbit pAb
Catalog No.A6163
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells. This gene and the related gene, leukemia inhibitory factor, also present on chromosome 22, may have resulted from the duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-252 of human Oncostatin M (NP_065391.1).
SequenceAAIGSCSKEYRVLLGQLQKQTDLMQDTSRLLDPYIRIQGLDVPKLREHCRERPGAFPSEETLRGLGRRGFLQTLNATLGCVLHRLADLEQRLPKAQDLERSGLNIEDLEKLQMARPNILGLRNNIYCMAQLLDNSDTAEPTKAGRGASQPPTPTPASDAFQRKLEGCRFLHGYHRFMHSVGRVFSKWGESPNRSRRHSPHQALRKGVRRTRPSRKGKRLMTRGQLPR
Gene ID
Swiss Prot
SynonymsOSM
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHepG2, NIH/3T3, Mouse uterus, Mouse heart
Cellular locationSecreted
Customer validation

WB(Mus musculus, Homo sapiens, Mus musculus)

IF(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - Oncostatin M Rabbit pAb (A6163)}

Western blot - Oncostatin M Rabbit pAb (A6163)

Western blot analysis of extracts of various cell lines, using Oncostatin M Antibody (A6163) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunofluorescence - Oncostatin M Rabbit pAb (A6163)}

Immunofluorescence - Oncostatin M Rabbit pAb (A6163)

Immunofluorescence analysis of C6 cells using Oncostatin M antibody (A6163) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Oncostatin M Rabbit pAb (A6163)}

Immunofluorescence - Oncostatin M Rabbit pAb (A6163)

Immunofluorescence analysis of L-929 cells using Oncostatin M antibody (A6163) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A6163? Please let us know so that we can cite the reference in this datasheet.

재조합 단백질 (2)

항체 (1)

Secondary Antibodies (26)