제품 > 항체 > 다중클론항체(pAb)

OsFD1 Rabbit pAb (A19175)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Oryza sativa

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsFD1 Rabbit pAb (A19175) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Overview

Product name	OsFD1 Rabbit pAb
Catalog No.	A19175
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Hd3a interacts with 14-3-3 proteins in the apical cells of shoots, yielding a complex that translocates to the nucleus and binds to the Oryza sativa (Os)FD1 transcription factor, a rice homologue of Arabidopsis thaliana FD. The resultant ternary 'florigen activation complex' (FAC) induces transcription of OsMADS15, a homologue of A. thaliana APETALA1 (AP1), which leads to flowering.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of Oryza sativa OsFD1. (A0A0P0XR34).
Sequence	MAMEDDEDMWANTSSPSASPPRPRGFISTALSLNSTHLQGLLPSSFVDAAASPCHASGNNNGGGDGRNAAPMSSIFFASASYHQQQHHLPAPAPLDGAILPARRFGLDMCAAAAAAPAGVPAAGD
Gene ID
Swiss Prot
Synonyms	P0478E02.1; OsFD1
Calculated MW	21kDa
Observed MW	21kDa

Applications

Reactivity	Oryza sativa
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	leaves, stems
Cellular location	nucleus
Customer validation	WB(Oryza sativa)