제품 > 항체 > 다중클론항체(pAb)

OsGI Rabbit pAb (A20671)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsGI Rabbit pAb (A20671)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsGI Rabbit pAb (A20671) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameOsGI Rabbit pAb
Catalog No.A20671
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in regulation of circadian rhythm, and in the control of the photoperiodic flowering. Acts as suppressor of flowering under short-day (SD) and long-day (LD) conditions. Activates Hd1/CONSTANS gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 820-1090 of Oryza sativa OsGI. (Q9AWL7).
SequenceALEKKDASRSHRKNGFARPLLKCAEDVILNGDVASTSGKAIASLQVEASDLANFLTMDRNGGYRGSQTLLRSVLSEKQELCFSVVSLLWQKLIASPEMQMSAESTSAHQGWRKVVDALCDIVSASPTKASAAIVLQAEKDLQPWIARDDEQGQKMWRVNQRIVKLIAELMRNHDSPEALVILASASDLLLRATDGMLVDGEACTLPQLELLEVTARAVHLIVEWGDSGVSVADGLSNLLKCRLSTTIRCLSHPSAHVRALSMSVLRDILNS
Gene ID
Swiss Prot
SynonymsGI; OsGI
Calculated MW127kDa
Observed MW100kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesleaves, stems
Cellular location
    ABclonal:Western blot - OsGI Rabbit pAb (A20671)}

    Western blot - OsGI Rabbit pAb (A20671)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsGI Rabbit pAb (A20671) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)