제품 > 항체 > 다중클론항체(pAb)

OsGRF4 Rabbit pAb (A20348)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsGRF4 Rabbit pAb (A20348)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsGRF4 Rabbit pAb (A20348) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameOsGRF4 Rabbit pAb
Catalog No.A20348
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Transcription activator that plays a role in the regulation of meristematic function in leaves, stems and inflorescences Transcription activator that plays a regulatory role in grain development. Positively regulates grain size by promoting cell division and expansion, leading to increased grain length and width. Positively regulates the expression of genes promoting cell proliferation. Activates the expression of expansin genes to promote cell expansion and grain size . May promote grain size by activating brassinosteroid responses. Component of a network formed by the microRNA396 (miRNA396), the GRFs and their interacting factors (GIFs) acting in the regulation of meristem function, at least partially through the control of cell proliferation (Probable). Component of the miRNA396c-GRF4-GIF1 regulatory module that plays an important role in grain size determination.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 120-394 of Oryza sativa OsGRF4. (Q6ZIK5).
SequenceFGKKLDPEPGRCRRTDGKKWRCSKEAAPDSKYCERHMHRGRNRSRKPVETQLVAQSQPPSSVVGSAAAPLAAASNGSSFQNHSLYPAIAGSNGGGGGRNMPSSFGSALGSQLHMDNAAPYAAVGGGTGKDLRYTAYGTRSLADEQSQLITEAINTSIENPWRLLPSQNSPFPLSSYSQLGALSDLGQNTPSSLSKVQRQPLSFFGNDYAAVDSVKQENQTLRPFFDEWPKGRDSWSDLADENANLSSFSGTQLSISIPMASSDFSAASSRSTNGD
Gene ID
Swiss Prot
SynonymsGL2; GS2; PT2; GLW2; GRF4; grf2; OsGRF4; OsJ_08054; OJ1111_E07.19
Calculated MW42kDa
Observed MW40kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesleaves (after flower), flowers, milk seeds
Cellular locationnucleus
Customer validation

IP(Triticum aestivum)

    ABclonal:Western blot - OsGRF4 Rabbit pAb (A20348)}

    Western blot - OsGRF4 Rabbit pAb (A20348)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsGRF4 Rabbit pAb (A20348) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20348? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)