제품 > 항체 > 다중클론항체(pAb)

OsNAC4 Rabbit pAb (A20653)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsNAC4 Rabbit pAb (A20653)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsNAC4 Rabbit pAb (A20653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameOsNAC4 Rabbit pAb
Catalog No.A20653
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
OsNAC4 is a key positive regulator of plant hypersensitive cell death
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 210-310 of Oryza sativa OsNAC4. (Q52QH4).
SequenceAAAADTMSDSFQTHDSDIDNASAGLRHGGCGGGGFGDVAPPRNGFVTVKEDNDWFTGLNFDELQPPYMMNLQHMQMQMVNPAAPGHDGGYLQSISSPQMKM
Gene ID
Swiss Prot
SynonymsNAC4; NAC68; NAC068; OsNAC4; ONAC068; B1148D12.1
Calculated MW35kDa
Observed MW40kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesspikes
Cellular locationnucleus
    ABclonal:Western blot - OsNAC4 Rabbit pAb (A20653)}

    Western blot - OsNAC4 Rabbit pAb (A20653)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsNAC4 Rabbit pAb (A20653) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)