제품 > 항체 > 다중클론항체(pAb)

OsRACK1B Rabbit pAb (A18595)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsRACK1B Rabbit pAb (A18595)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsRACK1B Rabbit pAb (A18595) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Overview

Product nameOsRACK1B Rabbit pAb
Catalog No.A18595
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Component of the RACK1 regulatory proteins that play a role in multiple signal transduction pathways.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of Oryza sativa OsRACK1B. (Q6L4F8).
SequenceMAGQESLTLAGVLRGHNDMVTAIAAPIDNSPFIVSSSRDKSLLVWDITNPSTAVATDPEAAPPEYGVSYRRLTGHSHFVQDVVLSSDGQFALSGSWDGEL
Gene ID
Swiss Prot
SynonymsGPB-LR; RACK1B; OsJ_19467; OsRACK1B
Calculated MW36kDa
Observed MW36kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesleaves, stems, spikes, milk grains
Cellular locationcytosol, nucleus
    ABclonal:Western blot - OsRACK1B Rabbit pAb (A18595)}

    Western blot - OsRACK1B Rabbit pAb (A18595)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsRACK1B Rabbit pAb (A18595) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)