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OsRDR6 Rabbit pAb (A20319)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - OsRDR6 Rabbit pAb (A20319)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsRDR6 Rabbit pAb (A20319) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameOsRDR6 Rabbit pAb
Catalog No.A20319
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
nvolved in the RNA silencing pathway. Probably required for the generation of small interfering RNAs (siRNAs). Regulates shoot apical meristem (SAM) initiation and maintenance and leaf polarization through the trans-acting siRNAS (ta-siRNAs) pathway which probably modulates the expression of the ARF2, ARF3, ARF4, ARF14 and ARF15 genes.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 963-1218 of Oryza sativa OsRDR6. (Q8LHH9).
SequenceMDEKCIHLAELAATAVDFPKTGKLAIMPPHLKPKVYPDFMGKEDGQSYKSEKILGRLYRSIQEASNGDVVSQEVCTPNDLPYDIDLEVPGASDFLASAWQCKCSYDAQLSALLSQYRVRTEAELVTGHITFLVKNSSKKQGDIKDRLKTAYSALRKEFKSTFESIASDQCEIGDDEKNLLYEMKASAWYQVTYHPKWVEKSRGILGPDGEEIPASLSFAWIPVDYLARIKLRCHGKVRVEGQKPVERLAAYISERI
Gene ID
Swiss Prot
SynonymsSHL2; SHOOTLESS2; OsRDR6
Calculated MW137kDa
Observed MW140kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesstems (before flower), leaves sheath (before flower), flower buds (before flower), flag leaves (after flower), flowers, milk seeds
Cellular location
Customer validation

WB(Oryza sativa)

    ABclonal:Western blot - OsRDR6 Rabbit pAb (A20319)}

    Western blot - OsRDR6 Rabbit pAb (A20319)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using OsRDR6 Rabbit pAb (A20319) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20319? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)